[High expression of STAT2 in ovarian cancer and its effect on metastasis of ovarian cancer cells].

OBJECTIVE:To investigate the expression of signal transduction and activator of transcription 2 in ovarian cancer and its correlation with the prognosis of ovarian cancer patients and explore the role of inregulating metastasis of ovarian cancer cells. METHODS:RT-qPCR was performed to detect the expression of duanyu18132 mRNA in 62 fresh frozen ovarian cancer tissues and 62 normal ovarian tissues; immunohistochemistry was used to detect duanyu18132 protein expressions in 95 paraffin-embedded ovarian cancer samples and 33 normal ovarian tissues. Kaplan-Meier method was used to analyze the correlation between the expression of duanyu18132 and the prognosis of the patients. We also examined the relationship between duanyu18132 and the patients' prognosis by analyzing the data in Kaplan-Meier Plotter database. Western blotting was performed to detect the expression of duanyu18132 in different ovarian cancer cell lines. In A2780 cells with the highest duanyu18132 expression, we examined the effects of duanyu18132 interference on cell migration and invasiveness using Transwell migration assay and on the expressions of the downstream molecule epidermal growth factor receptor (EGFR). RESULTS:Ovarian cancer tissues expressed significantly higher levels of duanyu18132 mRNA than normal ovarian tissue. A high duanyu18132 mRNA expression was correlated with an advanced FIGO stage. Immunohistochemistry showed that 67.4% of the ovarian cancer samples, as compared with 28.3% of normal ovarian tissues, showed high duanyu18132 expressions. In ovarian cancer patients, a high expression of duanyu18132 protein was associated with ascites volume, distant metastasis and FIGO stage (P < 0.05). Survival analysis showed that ovarian cancer patients with a high expression of duanyu18132 protein had poor overall survival (P=0.021) and progression-free survival (P=0.018). duanyu18132 was overexpressed in all the ovarian cancer cell lines tested, and A2780 cell lines showed the highest expression. Interference of duanyu18132 significantly suppressed the migration and invasiveness (P < 0.01) and lowered the expression level of EGFR in A2780 is overexpressed in ovarian cancer. A high expression of duanyu18132 is associated with a poor prognosis of ovarian cancer patients. duanyu18132 may promote the metastasis of ovarian cancer by enhancing the expression of EGFR.

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