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Upregulation of DAB2IP Inhibits Ras Activity and Tumorigenesis in Human Pancreatic Cancer Cells.

Technol. Cancer Res. Treat.2020 Jan-Dec ;19:1533033819895494. doi:10.1177/1533033819895494
Yifan Duan 1 , Xiaoyu Yin 2 , Xiaorong Lai 3 , Chao Liu 4 , Wenjing Nie 5 , Dongfeng Li 6 , Zijun Xie 7 , Zijun Li 8 , Fan Meng 9
Yifan Duan 1 , Xiaoyu Yin 2 , Xiaorong Lai 3 , Chao Liu 4 , Wenjing Nie 5 , Dongfeng Li 6 , Zijun Xie 7 , Zijun Li 8 , Fan Meng 9
+ et al

[No authors listed]

Author information
  • 1 Department of Gastroenterology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
  • 2 Department of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
  • 3 Department of Oncology Medicine, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
  • 4 Department of Pathology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
  • 5 The Third People's Hospital of Foshan, Foshan City, Guangdong Province, China.
  • 6 Research Center of Medical Sciences, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
  • 7 Department of Gastroenterology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou City, Guangdong Province, China.
  • 8 Guangdong Provincial Institute of Geriatrics, Guangzhou, China.
  • 9 Department of Gastroenterology, The First Affiliated Hospital of Gannan Medical University, Ganzhou, China.

摘要

KRAS mutation-induced Ras activation plays an important role in the pathogenesis of pancreatic cancer, but the role of wild-type Ras and Ras GTPase-activating proteins remains unclear. The present study was designed to determine the expression spectra of Ras GTPase-activating proteins genes in pancreatic cancer cells, and the role of DAB2IP, a Ras GTPase-activating proteins gene, in the development and progression of pancreatic cancer. Following the analyses of the expression profiles of 16 Ras GTPase-activating proteins in 6 pancreatic cancer cell lines including Bxpc-3 (with wild-type KRAS), Capan-2, Sw1990, Aspc-1, CFPAC-1, and Panc-1 (with mutant KRAS) and 1 normal human pancreatic ductal epithelial cell line, H6C7, the expression of DAB2IP messenger RNA was further analyzed by quantitative real-time polymerase chain reaction. The role of DAB2IP in pancreatic cancer was further investigated in vitro and in vivo by upregulating DAB2IP in Bxpc-3 cells through transfection of DAB2IP into Bxpc-3 cells with recombinant lentivirus. The DAB2IP expression in pancreatic cancer cells and tissues with wild-type KRAS was significantly lower than that in cells and tissues with mutant KRAS (P < .05). In Bxpc-3 cells with wild-type KRAS, overexpression of DAB2IP decreased the expression of P-AKT and P-ERK and the Ras activity; increased the expression of P-JNK and caspase 3; inhibited cell proliferation, invasiveness, and migration; and increased the cell sensitivity to cetuximab. Overexpression of DAB2IP inhibited tumor progression in a mouse model. In conclusion, DAB2IP downregulates Ras activity in wild-type pancreatic cancer cells. Overexpression of DAB2IP decreases the Ras activity, inhibits cell proliferation, and increases sensitivity to cetuximab in wild-type pancreatic cancer cells. In conclusion, DAB2IP may serve as a potential molecular therapeutic target for the treatment of pancreatic cancer.

KEYWORDS: Bxpc-3 cells, DAB2IP, Ras activity, RasGAP, pancreatic cancer, tumor suppressors

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