The association between ¹¹C-methionine uptake, IDH gene mutation, and MGMT promoter methylation in patients with grade II and III gliomas.

AIM:To evaluate the association between ¹¹C-methionine positron-emission tomography (¹¹C-methionine PET) findings, isocitrate dehydrogenase (IDH) gene mutation, and O⁶-methylguanine-DNA methyltransferase (MGMT) promoter methylation in patients with grade II and III gliomas. MATERIALS AND METHODS:Data were collected from 40 patients with grade II and III gliomas who underwent both magnetic resonance imaging (MRI) and ¹¹C-methionine PET as part of their pre-surgical examination. IDH mutation was examined via DNA sequencing, and MGMT promoter methylation via quantitative methylation-specific polymerase chain reaction (PCR). RESULTS:A threshold of MGMT promoter methylation of 1% was significantly associated with tumour/normal tissue (T/N) ratio. The T/N ratio in samples with MGMT promoter methylation ≥1% was higher than that in samples with MGMT promoter methylation <1%, and the difference was statistically significant (p=0.011). Reliable prediction of MGMT promoter methylation (<1% versus ≥1%) was possible using the T/N ratio under the receiver operator characteristic (ROC) curve with a sensitivity and specificity of 75% each (cut-off value=1.6: p=0.0226, area under the ROC curve [AUC]=0.76172). Conversely, the T/N ratio had no association with IDH mutation (p=0.6). The ROC curve revealed no reliable prediction of IDH mutation using the T/N ratio (p=0.606, AUC=0.60577). CONCLUSION:¹¹C-methionine PET parameters can predict MGMT promoter methylation but not IDH mutation status. ¹¹C-methionine uptake may have limited potential to reflect DNA methylation processes in grade II and III gliomas.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}