[No authors listed]
First discovered as an oncogene in leukaemia, recent reports highlight an emerging role for the protoâoncogene BCLâ3 in solid tumours. Importantly, BCLâ3 expression is upregulated in >30% of colorectal cancer cases and is reported to be associated with a poor prognosis. However, the mechanism by which BCLâ3 regulates tumorigenesis in the large intestine is yet to be fully elucidated. In the present study, it was shown for the first time that knocking down BCLâ3 expression suppressed cyclooxygenaseâ2 (COXâ2)/prostaglandin E2 (PGE2) signalling in colorectal cancer cells, a pathway known to drive several of the hallmarks of cancer. suppression of BCLâ3 expression decreased COXâ2 expression in colorectal cancer cells both at the mRNA and protein level. This reduction in COXâ2 expression resulted in a significant and functional reduction (30â50%) in the quantity of proâtumorigenic PGE2 produced by the cancer cells, as shown by enzyme linked immunoassays and medium exchange experiments. In addition, inhibition of BCLâ3 expression also significantly suppressed cytokineâinduced (TNFâα or ILâ1β) COXâ2 expression. Taken together, the results of the present study identified a novel role for BCLâ3 in colorectal cancer and suggested that expression of BCLâ3 may be a key determinant in the COXâ2âmeditated response to inflammatory cytokines in colorectal tumour cells. These results suggest that targeting BCLâ3 to suppress PGE2 synthesis may represent an alternative or complementary approach to using nonâsteroidal antiâinflammatory drugs [(NSAIDs), which inhibit cyclooxygenase activity and suppress the conversion of arachidonic acid to prostaglandin], for prevention and/or recurrence in PGE2âdriven tumorigenesis.
KEYWORDS: {{ getKeywords(articleDetailText.words) }}
Sample name | Organism | Experiment title | Sample type | Library instrument | Attributes | |||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
{{attr}} | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
{{ dataList.sampleTitle }} | {{ dataList.organism }} | {{ dataList.expermentTitle }} | {{ dataList.sampleType }} | {{ dataList.libraryInstrument }} | {{ showAttributeName(index,attr,dataList.attributes) }} |
{{ list.authorName }} {{ list.authorName }} |