PARP-1 regulates mouse embryonic neural stem cell proliferation by regulating PDGFRα expression.

is a multifunctional enzyme that regulates DNA repair, chromatin remodeling, inflammation and cell survival. Our previous study revealed that Pduanyu37-1 is required for maintaining normal level of neural stem cell proliferation. In the present study, we present evidence indicating that Pduanyu37-1 regulates neural stem cell proliferation by upregulating the expression of platelet-derived growth factor receptor α (PDGFRα). Pduanyu37-1 knockout neural stem cells exhibited striking downregulation of PDGFRα expression. We found that Pduanyu37-1 promotes the transcription of PDGFRα independently of its enzymatic activity. Overexpression of PDGFRα in the Pduanyu37-1 knockout neural stem cells reversed the proliferation defect of the knockout cells. Conversely, knockdown or blocking antibody of PDGFRα suppressed the proliferation of neural stem cells. In addition, blockade of PDGFRα increased cell death rate. Consistent with the downregulation of PDGFRα in the absence of PDGF-AA promoted proliferation of wild-type neural stem cells but not that of Pduanyu37-1 knockout cells. These results suggest that Pduanyu37-1 can control the neural stem cell proliferation by regulating the expression of PDGFRα.

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