例如:"lncRNA", "apoptosis", "WRKY"

PGRMC1 effects on metabolism, genomic mutation and CpG methylation imply crucial roles in animal biology and disease.

BMC Mol Cell Biol. 2020 Apr 15;21(1):26
Bashar M Thejer 1 , Partho P Adhikary 2 , Sarah L Teakel 3 , Johnny Fang 3 , Paul A Weston 4 , Saliya Gurusinghe 5 , Ayad G Anwer 6 , Martin Gosnell 7 , Jalal A Jazayeri 3 , Marina Ludescher 8 , Lesley-Ann Gray 9 , Michael Pawlak 10 , Robyn H Wallace 3 , Sameer D Pant 11 , Marie Wong 12 , Tamas Fischer 13 , Elizabeth J New 14 , Tanja N Fehm 8 , Hans Neubauer 8 , Ewa M Goldys 6 , Jane C Quinn 15 , Leslie A Weston 4 , Michael A Cahill 16
Bashar M Thejer 1 , Partho P Adhikary 2 , Sarah L Teakel 3 , Johnny Fang 3 , Paul A Weston 4 , Saliya Gurusinghe 5 , Ayad G Anwer 6 , Martin Gosnell 7 , Jalal A Jazayeri 3 , Marina Ludescher 8 , Lesley-Ann Gray 9 , Michael Pawlak 10 , Robyn H Wallace 3 , Sameer D Pant 11 , Marie Wong 12 , Tamas Fischer 13 , Elizabeth J New 14 , Tanja N Fehm 8 , Hans Neubauer 8 , Ewa M Goldys 6 , Jane C Quinn 15 , Leslie A Weston 4 , Michael A Cahill 16
+ et al

[No authors listed]

Author information
  • 1 Department of Biology, College of Science, University of Wasit, Kut, Wasit, Iraq.
  • 2 Present Address: Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, Canada.
  • 3 School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, 2650, Australia.
  • 4 School of Agricultural and Wine Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, NSW, 2678, Australia.
  • 5 Graham Centre for Agricultural Innovation, Charles Sturt University, Boorooma Street, Wagga Wagga, NSW, 2678, Australia.
  • 6 Present Address: The Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Kensington, NSW, 2052, Australia.
  • 7 Quantitative (Biotechnology) Pty. Ltd., ABN 17 165 684 186, Australia.
  • 8 Department of Gynecology and Obstetrics, University Women's Hospital of Dusseldorf, Dusseldorf, Germany.
  • 9 Australian Genome Research Facility Ltd., Victorian Comprehensive Cancer Centre, Melbourne, VIC, 3000, Australia.
  • 10 NMI TT Pharmaservices, Protein Profiling, 72770 Reutlingen, Germany.
  • 11 School of Animal and Veterinary Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, NSW, 2678, Australia.
  • 12 Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Darlinghurst, NSW, 2010, Australia.
  • 13 ACRF Department of Cancer Biology and Therapeutics, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT, 2601, Australia.
  • 14 University of Sydney, School of Chemistry, Sydney, NSW, 2006, Australia.
  • 15 Faculty of Science, Charles Sturt University, Boorooma Street, Wagga Wagga, NSW, 2678, Australia.
  • 16 ACRF Department of Cancer Biology and Therapeutics, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT, 2601, Australia. mcahill@csu.edu.au.

摘要


BACKGROUND:Progesterone receptor membrane component 1 (PGRMC1) is often elevated in cancers, and exists in alternative states of phosphorylation. A motif centered on PGRMC1 Y180 was evolutionarily acquired concurrently with the embryological gastrulation organizer that orchestrates vertebrate tissue differentiation. RESULTS:Here, we show that mutagenic manipulation of PGRMC1 phosphorylation alters cell metabolism, genomic stability, and CpG methylation. Each of several mutants elicited distinct patterns of genomic CpG methylation. Mutation of S57A/Y180/S181A led to increased net hypermethylation, reminiscent of embryonic stem cells. Pathways enrichment analysis suggested modulation of processes related to animal cell differentiation status and tissue identity, as well as cell cycle control and ATM/ATR DNA damage repair regulation. We detected different genomic mutation rates in culture. CONCLUSIONS:A companion manuscript shows that these cell states dramatically affect protein abundances, cell and mitochondrial morphology, and glycolytic metabolism. We propose that PGRMC1 phosphorylation status modulates cellular plasticity mechanisms relevant to early embryological tissue differentiation.

KEYWORDS: Cell death, Cytochrome P450, Embryology, Epigenetics, Genomic sequence, Hyperspectral autofluorescence, Metabolism, Organizer, Steroid biology