[No authors listed]
Galectin-3 binding protein (LGALS3BP or 90âK) is a secreted glycoprotein found in human body fluids. Deregulated levels were observed in cancer and infection and its study in neurological diseases is more recent. Here, we have investigated 90âK from human cerebrospinal fluid (CSF) of patients with amyotrophic lateral sclerosis (ALS, nâ=â35) and other neurological diseases (nâ=â23). CSF was fractionated by ultrafiltration/size-exclusion chromatography (SEC) and eluted fractions were analysed by complementary techniques including immunoblotting, electron microscopy and nano-liquid chromatography-tandem mass spectrometry. A fraction of 90âK appeared as nanoparticles of irregular shape with heterogeneous dimensions of 15-60ânm that co-eluted with extracellular vesicles in SEC. Median levels of 90âK quantified by ELISA were not different between ALS patients (215.8âng/ml) and controls (213.3âng/ml) in contrast with the benchmark biomarker for ALS phosphoneurofilament heavy chain (1750 and 345âpg/ml, respectively). A multiregression model supported age is the only independent predictor of 90âK level in both groups (pâ<â0.05). Significant correlation was found between 90âK levels and age for the ALS group (râ=â0.366, pâ=â0.031) and for all subjects (râ=â0.392, pâ=â0.003). In conclusion, this study unveils the presence of 90 K-containing nanoparticles in human CSF and opens novel perspectives to further investigate 90âK as potential aging marker.
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