[No authors listed]
MicroRNAs (miRNAs/miRs) are non-coding RNAs that regulate protein synthesis by targeting mRNAs for translational repression or degradation. Previous studies have reported that aberrant expression of miRâ744 may be involved in human osteosarcoma; however, the underlying mechanisms remain elusive. In the present study, the expression levels of miRâ744 and its downstream signals were determined by reverse transcriptionâquantitative PCR and western blotting. Cell proliferation was assessed using the bromodeoxyuridine assay, and the target of miRâ744 was investigated using a dualâluciferase activity assay. The present study identified a significant upregulation of miRâ744 in osteosarcoma tissues compared with adjacent nonâtumor tissues. Furthermore, it was demonstrated that ectopic overexpression of miRâ744 induced by a miRâ744 precursor significantly enhanced proliferation of the osteosarcoma cell line MG63, whereas opposite results were observed following suppression of miRâ744 with its inhibitor. Moreover, as a unique antiâoncogene, PTEN was identified as a direct target of miRâ744. It was confirmed that miRâ744 downregulated PTEN expression in MG63 cells by targeting the PTEN 3'untranslated region, and that the downstream AKT signal was also regulated by miRâ744. Collectively, the present results suggested that miRâ744 promoted proliferation of human osteosarcoma cells by directly regulating the PTEN/AKT signaling pathway.
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