例如:"lncRNA", "apoptosis", "WRKY"

miR-29c-3p inhibits microglial NLRP3 inflammasome activation by targeting NFAT5 in Parkinson's disease.

Genes Cells. 2020 Jun;25(6):364-374. doi:10.1111/gtc.12764. Epub 2020 Mar 30
Ruili Wang 1 , Qing Li 1 , Ya He 1 , Ying Yang 1 , Qiaoya Ma 1 , Chen Li 1
Ruili Wang 1 , Qing Li 1 , Ya He 1 , Ying Yang 1 , Qiaoya Ma 1 , Chen Li 1
+ et al

[No authors listed]

Author information
  • 1 Department of Geriatric Neurology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

摘要


Microglial inflammation is identified as a key process associated with Parkinson's disease (PD) pathogenesis. Our previous study showed that miR-29c-3p (miR-29c) exhibited anti-inflammatory properties in PD animal and neuronal models. However, the specific role and regulatory mechanism of miR-29c played in microglia are still unclear. In this study, lipopolysaccharide (LPS)-stimulated BV-2 cells were used to establish a cellular model of microglial activation for investigating PD. The results showed a decreased expression of miR-29c in LPS-induced BV-2 cells. Over-expression of miR-29c suppressed LPS-triggered Iba-1 increment, pro-inflammatory cytokine release, and NF-кB and TXNIP/NLRP3 inflammasome activation. Silence of miR-29c induced similar effects with LPS on microglial inflammation. In addition, we found that NFAT5 was negatively correlated with miR-29c. Knockdown of NFAT5 blocked the aggravated inflammation in microglia treated by miR-29c inhibitor. Thus, these findings suggest that miR-29c modulates NLRP3 inflammasome to impair microglial inflammatory responses by targeting NFAT5, which represents a promising therapeutic target for PD.

KEYWORDS: NFAT5, NLRP3 inflammasome, Parkinson's disease, miR-29c-3p, microglia