Circular RNA_LARP4 inhibits the progression of non-small-cell lung cancer by regulating the expression of SMAD7.

OBJECTIVE:Researchers have uncovered the importance of circular RNAs (circ) in malignant tumors. Circ has been found to serve as a tumor suppressor gene in gastric cancer. However, the exact function of circ Lduanyu374 in non-small-cell lung cancer (NSCLC) has not been fully elucidated. The aim of this study was to uncover the role of circ Lduanyu374 in the tumorigenesis of NSCLC. PATIENTS AND METHODS:Expression level of circ Lduanyu374 in NSCLC tissues was detected through Real Time-quantitative (RT-qPCR). Subsequently, the association between expression and patients' prognosis was analyzed. Circ Lduanyu374 lentivirus was constructed and transfected into NSCLC cells. The effect of circ Lduanyu374 on NSCLC cell migration and invasion was detected by function assays. Furthermore, Western blot was performed to analyze the expression of predicted protein of circ with adjacent tissues, circ Lduanyu374 was lowly expressed in NSCLC tissues. Meanwhile, expression of circ Lduanyu374 was associated with the prognosis of NSCLC patients. Downregulated circ Lduanyu374 was found in NSCLC cell lines as well. The migration and invasion abilities of NSCLC cells were significantly inhibited via overexpression of circ SMAD7, the predicted protein of circ increased remarkably via overexpression of circ Lduanyu374 could suppress the metastasis of NSCLC by up-regulating SMAD7.

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