Lin28B/miR-92b Promote the Proliferation, Migration, and Invasion in the Pathogenesis of Preeclampsia via the DKK1/Wnt/β-Catenin Pathway.

Preeclampsia (PE) is a disease unique to pregnancy and one of the leading causes of maternal and neonatal morbidity and mortality. Our previous study found that Lin28b, an RNA-binding protein stem cell factor, is down-expressed in the placenta of preeclampsia and significantly increases the invasion of HTR-8/SVneo cells in vitro. However, the mechanism of Lin28b's role is unclear. The purpose of this study is to investigate whether Lin28B affects the biological behavior and vascular development of trophoblast cells through miR-92b and downstream signaling pathway DKK1/Wnt/β-catenin. Our study demonstrated that Lin28B promotes trophoblast invasion through miR-92b in HTR-8 cells. Further experiments showed that microRNA-92b could negatively regulate DKK1 expression in placental trophoblasts, thereby inhibiting the activity of Wnt/beta-catenin signaling pathway, thereby inhibiting the migration and invasion of trophoblasts. Furthermore, we explored the expression of DKK1 and β-catenin in the placental tissues of preeclampsia patients and 20 healthy people. This study confirmed that Lin28 acts on DKK1 through miR-92b, which affects the expression of downstream Wnt/β-catenin, inhibits the invasion of trophoblast cells and the development of placental vasculature, and participates in the occurrence of PE.

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