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Testicular expression of TDRD1, TDRD5, TDRD9 and TDRD12 in azoospermia.

BMC Med. Genet.2020 Feb 14;21(1):33
Emad Babakhanzadeh 1 , Ali Khodadadian 2 , Saadi Rostami 3 , Iraj Alipourfard 4 , Mohsen Aghaei 2 , Majid Nazari 2 , Mehdi Hosseinnia 5 , Mohammad Yahya Vahidi Mehrjardi 1 , Yalda Jamshidi 6 , Nasrin Ghasemi 7
Emad Babakhanzadeh 1 , Ali Khodadadian 2 , Saadi Rostami 3 , Iraj Alipourfard 4 , Mohsen Aghaei 2 , Majid Nazari 2 , Mehdi Hosseinnia 5 , Mohammad Yahya Vahidi Mehrjardi 1 , Yalda Jamshidi 6 , Nasrin Ghasemi 7
+ et al

[No authors listed]

Author information
  • 1 Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
  • 2 Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
  • 3 Department of Cellular and Molecular Biology, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, Iran.
  • 4 School of Pharmacy, Faculty of Sciences, University of Rome Tor Vergata, Rome, Italy.
  • 5 Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
  • 6 Genetics Centre, Molecular and Clinical Sciences Institute, St George's University of London, London, UK.
  • 7 Abortion Research Centre, Yazd Reproductive Sicences Institue, Shahid sadoughi University of Medical Sciences, Yazd, Iran. nghasemi479@gmail.com.

摘要

BACKGROUND:Tudor domain-containing proteins (TDRDs) play a critical role in piRNA biogenesis and germ cell development. piRNAs, small regulatory RNAs, act by silencing of transposons during germline development and it has recently been shown in animal model studies that defects in TDRD genes can lead to sterility in males. METHODS:Here we evaluate gene and protein expression levels of four key TDRDs (TDRD1, TDRD5, TDRD9 and TDRD12) in testicular biopsy samples obtained from men with obstructive azoospermia (OA, n = 29), as controls, and various types of non-obstructive azoospermia containing hypospermatogenesis (HP, 28), maturation arrest (MA, n = 30), and Sertoli cell-only syndrome (SCOS, n = 32) as cases. One-way ANOVA test followed by Dunnett's multiple comparison post-test was used to determine inter-group differences in TDRD gene expression among cases and controls. RESULTS:The results showed very low expression of TDRD genes in SCOS specimens. Also, the expression of TDRD1 and TDRD9 genes were lower in MA samples compared to OA samples. The expression of TDRD5 significantly reduced in SCOS, MA and HP specimens than the OA specimens. Indeed, TDRD12 exhibited a very low expression in HP specimens in comparison to OA specimens. All these results were confirmed by Western blot technique. CONCLUSION:TDRDs could be very important in male infertility, which should be express in certain stages of spermatogenesis.

KEYWORDS: Non-obstructive Azoospermia, Spermatogenesis, TDRD genes, piRNAs

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