miR-3175 and miR-134 affect proliferation, invasion and apoptosis of glioma cells through PI3K/AKT signaling pathway.

PURPOSE:This study aimed to investigate the expressions of microRNA (miR)-3175 and miR-134 in gliomas and their effects on the proliferation and invasion of U251 glioma cells. METHODS:Tumor tissues of 42 patients with glioma and non-tumor tissues of 10 patients with severe craniocerebral injury were collected. These patients were diagnosed in Jinan City People's Hospital from March 2010 to April 2012. qRT-PCR was used to detect differences of miR-3175 and miR-134 expressions. Kaplan-Meier method was used to generate survival curves and Log-rank test to evaluate differences between miR-3175, miR-134 and 5-year survival of the patients. Western blot was used to detect levels of p-PI3K in PI3K signaling pathway. RESULTS:Proliferative activity of the cells in miR-3175 mimic group and miR-control group at different time points was significantly better than that of the cells in miR-3175 inhibitor group (p<0.05). The proliferative activity of the cells in miR-134 mimic group and miR-control group at different time points was significantly worse than that of the cells in miR-134 inhibitor group (p<0.05). Apoptosis rate of the cells in miR-3175 mimic group and miR control group was significantly lower than that of the cells in miR-3175 inhibitor group (p<0.05). Apoptosis rates of the cells in miR-134 mimic group and miR-control group were significantly higher than that of the cells in miR-134 inhibitor group (p<0.05). CONCLUSION:Downregulating expression of miR-3175 or facilitating expression of miR-134 could inhibit the proliferative and invasive activity of glioma cells and facilitate their apoptosis by inhibiting the activation of PI3K signaling pathway.

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