Activation of STAT-3 signalling by RECK downregulation via ROS is involved in the 27-hydroxycholesterol-induced invasion in breast cancer cells.

Breast cancer is an important and common tumour among women worldwide. We previously showed that 27-hydroxycholesterol (27HC) promoted the invasion and migration of breast cancer cells and activated signal transducer and activator of transcription 3 signalling through reactive oxygen species However, the regulation of signalling by needs to be further explored. Here, we showed that 27HC caused the accumulation of cellular which upregulated matrix metalloproteinase 9 (MMP9) and increased the invasive ability of MCF7 and T47D cells. 27HC decreased the protein and mRNA levels of reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) in a time- and dose-dependent manner in MCF7 and T47D cells. RECK downregulation was mediated by 27HC-induced DNA methylation via duanyu1670 in MCF7 cells. RECK knockdown increased the activity and mRNA levels of MMP9, and promoted the invasion of MCF7 cells. We also found RECK knockdown upregulated the level of in MCF7 cells. Furthermore, overexpression of RECK attenuated 27HC-induced invasion in MCF7 cells. RECK overexpression also inhibited p-duanyu1813-3 upregulation induced by 27HC. Collectively, the results showed that DNA methylation induced by 27HC via duanyu1670 downregulated RECK, thereby activating the duanyu1813-3 signalling pathway. RECK could serve as a novel target mediating the effect of 27HC on breast cancer.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}