[No authors listed]
The main purpose of this study was to evaluate the immunity, antioxidant indices, and disease resistance of quercetin in zebrafish (Danio rerio). A total of 630 fish were assigned to 21 tanks with 30 fish/tank, and they were exposed to 0, 0.01, 0.1, 1, 10, 100, and 1000 μg/L quercetin, respectively, for 56 days. Results indicated that the immune indices including acid phosphatase (ACP), myeloperoxidase (MPO), lysozyme activities, and Complement 3 (C3), C4, IgM contents were significantly higher in 1 μg/L quercetin group than these parameters in the control group (Pâ<â0.05). TNF-α and IL-8 mRNA expressions significantly decreased as the levels of quercetin increased up to 1 μg/L and increased thereafter (Pâ<â0.05). 1 and 10 μg/L quercetin groups showed significantly lower TNF-α and IL-8 mRNA levels than the quercetin-free group. Transforming growth factor-β and IL-10 mRNA levels showed an obviously opposite trend with TNF-α expression. The SOD, GPX, CAT, T-AOC activities, and SOD and GPX gene expression in the liver were enhanced with increasing quercetin up to 1 μg/L, and decreased thereafter. MDA contents were affected by quercetin, in which 1 and 10 μg/L quercetin had a significantly lower level than that of the control group (Pâ<â0.05). Defensin and Leap-II mRNA expression in the liver were the highest for fish exposed to 1 μg/L quercetin. The fish that exposed to 1 μg/L quercetin also showed a significantly higher survival rate than these of fish exposed to 0, 0.01, and 1000 μg/L quercetin (Pâ<â0.05). In conclusion, the optimal level of quercetin promotes immunostimulant properties, antioxidant indices, and disease resistance of zebrafish.
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