[No authors listed]
Parkinson's disease (PD) is the second most common neurodegenerative disorder. miRâ384â5p expression has been shown to be increased in an in vitro model of PD; however, it remains unknown whether there are other molecules that can be regulated by miRâ384â5p in in vivo and in vitro models of PD; thus, the present study aimed to elucidate this matter. Rotenone was applied for the establishment of in vitro and in vivo models of PD in the present study. Motor disability and equilibrium were determined by a swimming test and traction test, respectively. mRNA and protein levels were detected by reverse transcriptionâquantitative polymerase chain reaction (RTâqPCR) and western blot analysis, respectively. The association between miRâ384â5p and Sirtuin 1 (SIRT1) expression was verified by dual luciferase reporter assay. The αâsynuclein aggregation was evaluated by immunofluorescence. The results from the in vitro model of PD demonstrated that, the mice in the PD group exhibited decreased scores in the swimming test and traction test, which were accompanied by increased αâsynuclein aggregation. In addition, the expression of miRâ384â5p, which targeted the 3'untranslated region (3'UTR) of SIRT1, was verified to be increased in mice and SHâSY5Y cells in the PD group, whereas SIRT1 exhibited the opposite changes. Moreover, increased mRNA and protein levels of p53 and FOXO1 were observed in mice and SHâSY5Y cells in the PD group. In addition, the SHâSY5Y cells in the PD group exhibited a higher cell apoptotic rate. On the whole, the findings of this study demonstrate that miRNAâ384â5p promotes the progression of PD by targeting SIRT1.
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