Effect of Pim-3 Downregulation on Proliferation and Apoptosis in Lung Adenocarcinoma A549 Cells.

OBJECTIVE:To study the effects of Pim-3 on lung adenocarcinoma A549 cells. A549 cells were divided into an untreated group (without any treatment), a scramble siRNA group (transfected with control siRNA), and a Pim-3-deficient group (transfected with Pim-3 siRNA). METHODS:Pim-3-deficient cells are the experimental sample, whereas scramble siRNA and untreated cells are the corresponding negative controls. Western blotting was performed to detect the expression levels of Pim-3 protein, the phosphorylation of signal transducer and activator of transcription 3 and the expression levels of downstream target genes (p21, cyclin D1, Bcl-2, and Bax). Flow cytometry was performed to analyze cell cycle and apoptosis. RESULTS:In the Pim-3-deficient group, Pim-3 was downregulated, the phosphorylation level decreased, the levels of cyclin D1 and Bcl-2 decreased, but the levels of p21 and Bax increased. Meanwhile, cell proliferation was significantly inhibited (P<0.05); specifically, the G0/G1-phase cell proportion increased, whereas the S-phase cell proportion decreased and the proportion of early apoptotic cells increased significantly (P<0.05). CONCLUSION:The downregulation of Pim-3 was closely related to the activation status of the lung duanyu18133 signaling pathway, mediated cell proliferation inhibition and induced apoptosis. © 2019 by the Association of Clinical Scientists, Inc.

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