例如:"lncRNA", "apoptosis", "WRKY"

MiR-491 functions as a tumor suppressor through Wnt3a/β-catenin signaling in the development of glioma.

Eur Rev Med Pharmacol Sci. 2019 Dec;23(24):10899-10907. doi:10.26355/eurrev_201912_19793
{{ author.authorName }}{{getOrganisationIndexOf(author)}} {{ author.authorName }}{{getOrganisationIndexOf(author)}}
{{ author.authorName }}{{getOrganisationIndexOf(author)}} {{ author.authorName }}{{getOrganisationIndexOf(author)}}
+ et al

[No authors listed]

Author information
  • {{index+1}} {{ organisation }}

摘要


OBJECTIVE:Glioma is the most frequent brain tumor that has high invasion and usually disperses to the whole brain through blood and basement membranes. MicroRNA-491 (miR-491) has been reported to have low expression and act as a tumor suppressor in several cancers. The Wnt/β-catenin signaling is a classic signaling pathway that participated in several biological processes. Our purpose was to detect the molecular mechanism of miR-491 in regulating the growth and metastasis of glioma. MATERIALS AND METHODS:Real Time-quantitative (RT-qPCR) was applied to calculate the mRNA level of miR-491 and target gene. The protein expression of special genes was assessed by Western blot. The proliferation and invasive abilities were measured by the Cell Counting Kit-8 (CCK-8) and transwell assays. The Kaplan-Meier method was conducted to evaluate the association between the expressions of miR-491 with the overall survival of glioma patients. RESULTS:We discovered that miR-491 was lowly expressed in glioma and downregulation of miR-491 predicted poor outcome of glioma patients. Similarly, a high expression of miR-491 suppressed the growth and metastasis in glioma cell line LN229. MiR-491 high expression inhibited the growth of glioma in a mouse xenograft model. Moreover, Wnt3a was a target gene of miR-491 and miR-491 mediated the invasion-mediated epithelial-mesenchymal transition (EMT) by regulating the expression of Wnt3a. Additionally, miR-491 regulated the proliferation through the Wnt/β-Catenin pathway by targeting Wnt3a. CONCLUSIONS:MiR-491 overexpression inhibited the proliferation through the Wnt3a/β-catenin pathway and invasion-mediated EMT in glioma. The newly identified miR-491/Wnt3a/β-catenin axis provides novel insight into the pathogenesis of glioma.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}

基因功能


  • {{$index+1}}.{{ gene }}

图表


原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
{{attr}}
{{ dataList.sampleTitle }}
{{ dataList.organism }} {{ dataList.expermentTitle }} {{ dataList.sampleType }} {{ dataList.libraryInstrument }} {{ showAttributeName(index,attr,dataList.attributes) }}

文献解读