Legumain-deficient macrophages promote senescence of tumor cells by sustaining JAK1/STAT1 activation.

Macrophages serve as the first line of communication between tumors and the rest of the immune system, and understanding the interplay between macrophage and tumor cells is essential for developing novel macrophage-based strategy against tumor. Here, we show that deletion of legumain in macrophages activates senescence of tumor cells. Macrophage derived IL-1β mediates the pro-senescent effect of Lgmn^-/- macrophages since blockage of IL-1β reverses the senescence phenotype in both a coculture model of macrophage and tumor cells and an orthotopic mouse model of breast cancer. Sustained activation of signaling and increased iNOS were found in the tumor cell-cocultured Lgmn^-/- macrophages, which were necessary for IL-1β expression and secretion. Applying a specific agonist mimics the inductive effect of legumain deletion on IL-1β expression in macrophages, and the effect can be blocked via inhibition of iNOS. Legumain and integrin αvβ3 interact to prevent duanyu18131 signaling in macrophages, and blockage of integrin αvβ3 stimulates duanyu18131 activation. Therapeutically, transplantation of bone marrow from Lgmn^-/- mice suppresses the malignant growth of tumor by upregulating tumor cell senescence. Therefore, our finding highlights legumain in macrophages as a potential therapeutic target for tumors.

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