[No authors listed]
OBJECTIVE:This investigation was intended to elucidate lncRNA-miRNA networks that could explain inflammation underlying sepsis progression. METHODS:In the first place, four kinds of mice models were established, namely, SHAM group (nâ¯=â¯30), trauma (TH) group (nâ¯=â¯30), lipopolysaccharide (LPS) group (nâ¯=â¯30) and THâ¯+â¯LPS group (nâ¯=â¯30). Their lung, spleen and liver tissues were gathered for determination of TNF-α, IL-6, IL-10 and MCP-1 levels. Furthermore, mouse mononuclear macrophage leukemia cell line (RAW264.7) was stimulated by LPS to establish inflammation cell models. Then si-NEAT1s, pcDNA3.1-NEAT1, miR-495-3p mimic, miR-495-3p inhibitor, miR-NC, miR-211 mimic and miR-211 inhibitor were, respectively, transfected into the cells, so as to observe the impacts of NEAT1, miR-495-3p and miR-211 on cytokine levels released by the cells. RESULTS:The survival condition of mice in the THâ¯+â¯LPS group was undesirable, in relative to mice in the LPS group and SHAM group (both Pâ¯<â¯0.05). High-level NEAT1 and low-level miR-495-3p/miR-211 were associated with poor survival of mice in the TH+LPS group (Pâ¯<â¯0.05). Additionally, the correlation between NEAT1/miR-495-3p/miR-211 level and cytokine level was the strongest among TH+LPS-treated mice, in comparison to mice treated by TH or LPS. Furthermore, up-regulation of NEAT1 level and down-regulation of miR-495-3p/miR-211 level could aggravate inflammation in LPS-treated RAW264.7 cells. The miR-495-3p and miR-211 herein, were both subjected to sponging of NEAT1, possibly affected inflammation responses in RAW264.7 cells, respectively, through modulating and PI3K/AKT signaling. CONCLUSION:LncRNA NEAT1 exhibited great potential sepsis diagnosis and treatment, considering its modifying axis and miR-211/PI3K/AKT axis in inflammation cell models.
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