Regulatory effect of MiR103 on proliferation, EMT and invasion of oral squamous carcinoma cell through SALL4.

OBJECTIVE:Oral squamous cell carcinoma (OSCC) is a common tumor of head and neck cancer. MiR-103 is involved in several tumors. However, the role and mechanism of miR103 in OSCC remain unclear. MATERIALS AND METHODS:Oral cancer Tca8113 cells were cultured in vitro and randomly divided into control group, miR-103 mimics group, and miR-103 inhibitor group, followed by analysis of miR-103 expression by Real Time-PCR, SALL4 expression by Real Time-PCR and Western blot, cell survival by MTT assay, and cell invasion by transwell chamber assay on tumor. Real Time-PCR was performed to measure MMP-9 and MMP-2 expression. Western blot was conducted to detect E-cadherin and Vimentin expression. The Dual-Luciferase reporter system validated the relationship between miR103 and SALL4. RESULTS:Transfection of miR-103 mimics into Tca8113 cells significantly upregulated miR-103 expression, decreased SALL4 mRNA and protein expression, inhibited proliferation and invasion of Tca8113 cell, downregulated MMP-9 and MMP-2 mRNA expression, increased E-cadherin, and decreased Vimentin protein expression (p<0.05). However, miR-103 inhibitor transfection down-regulated miR-103 expression, promoted proliferation and invasion of Tca8113 cells, increased MMP-9 and MMP-2 mRNA expression, decreased E-cadherin expression, and elevated Vimentin expression. Compared with the control group, the differences were statistically significant (p<0.05). The Dual-Luciferase reports confirmed a targeted relationship between miR103 and SALL4. CONCLUSIONS:The overexpression of miR-103 inhibits MMP-9 and MMP-2 expression by negatively regulating SALL4, inhibiting proliferation and invasion of oral squamous cell carcinoma Tca8113 cells.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}