miR‑337‑3p inhibits gastric tumor metastasis by targeting ARHGAP10.

Several microRNAs (miRNAs) are known as regulatory molecules involved in gastric tumor metastasis. The expression of miR‑337‑3p was revealed to be downregulated in metastatic gastric tumor cells. Overexpression of miR‑337‑3p in gastric cancer cells resulted in the reduction of their invasive abilities. To characterize the functions of miR‑337‑3p, miR‑337‑3p was expressed in a metastatic lymph node‑derived gastric tumor cell line, SGC‑7901. Overexpression of miR‑337‑3p reduced the viability of cells but had no effects on the cell cycle. Wound healing and Transwell migration assays revealed that miR‑337‑3p inhibited the migration capacity of cells. miR‑337‑3p was capable of binding to the 3'‑untranslated region of a cytoskeleton‑associated molecule, ARHGAP10. Overexpression of miR‑337‑3p reduced the mRNA and protein levels of ARHGAP10 and the co‑expression of ARHGAP10 and miR‑337‑3p resulted in the recovery of cell migration capacity. Furthermore, the injection of miR‑337‑3p‑overexpressing SGC‑7901 cells into an immunodeficient mouse model resulted in a decrease in tumor metastasis in the liver and lungs. The present results indicated that miR‑337‑3p regulates gastric tumor metastasis by targeting the cytoskeleton‑associated protein ARHGAP10.

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