[No authors listed]
It has been universally acknowledged that interleukinâ37 (ILâ37) has an immunosuppressive effect on various inflammatory disorders. However, whether ILâ37 participates in the acute inflammation associated with coronary heart disease (CHD) has not yet been clarified. In the present study, the association between the serum levels of ILâ37 and the clinical indexes of CHD were analysed. In addition, the antiâinflammatory effects of ILâ37 on peripheral blood mononuclear cells (PBMCs) were studied in CHD patients. PBMCs from 46 healthy controls (HCs) and 92 CHD patients were cultured in vitro and stimulated using the recombinant ILâ37 protein. The protein levels, as well as the mRNA expression of inflammatory cytokines (TNFâα, ILâ1β, ILâ6, and ILâ17) were analysed by enzymeâlinked immunosorbent assay (ELISA) and realâtime polymerase chain reaction (RTâPCR). Spearman's correlation test was performed to examine the association between the serum level of ILâ37 and the levels of proâinflammatory cytokines, certain clinical indexes, and disease activity during CHD. Compared to the HCs, the CHD patients, especially those with acute myocardial infarction, exhibited higher levels of ILâ37 in their PBMCs and sera. Serum levels of ILâ37 were associated with the levels of ILâ17, ILâ6, and TNFâα, and clinical indexes such as the left ventricular ejection fraction (LVEF), aminoâNâterminal proâplasma brain natriuretic peptide (NTâproBNP) levels, and cardiac troponin T (cTnT) levels in CHD patients. Compared to the HC group, the production of inflammatory cytokines such as ILâ17, ILâ6, TNFâα, and ILâ1β increased in the PBMCs of CHD patients and significantly decreased after the stimulation of the cells with recombinant ILâ37. The ILâ37 levels in CHD patients were high, and were correlated with the levels of CHDârelated proâinflammatory cytokines and disease activity. Notably, the expression of CHDârelated proâinflammatory cytokines in the PBMCs of CHD patients decreased following the stimulation of the cells with recombinant ILâ37, indicating that ILâ37 exerts antiâinflammatory effects during CHD.
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