SPARC expression in desmoplastic and non desmoplastic pancreatic carcinoma and cholangiocarcinoma.

BACKGROUND:The pancreatobiliary carcinomas are characterized by presence of desmoplastic stroma. Overexpression of secreted protein acid and rich in cysteine a matrix producing agent has been documented in pancreatic ductal adenocarcinomas, with survival benefits. This study was targeted to see if expression in pancreatobiliary carcinomas is responsible for stromal desmoplasia and its prognostic significance. METHODS:In this retrospective study 48 cases of pancreatic cancer and 27 cases of cholangiocarcinoma were analyzed. The expression pattern of duanyu1842RC and vascular endothelial growth factor (VEGF) (angiogenic factors) was evaluated by immunohistochemistry on formalin fixed paraffin embedded tissues. Immunoreactivity was scored semi quantitatively based on stain intensity and stain distribution. duanyu1842RC expression was correlated with tumor histology, stromal desmoplasia, VEGF expression, various histological parameters and overall survival in patients. Real time polymerase chain reaction was performed in few cases to validate the immunohistochemistry expression expression was high in peritumoral stroma in pancreatic carcinoma than in pancreatic controls; however, duanyu1842RC expression pattern was not grossly different in desmoplastic and non-desmoplastic pancreatobiliary carcinomas and in cholangiocarcinomas. No definite correlation was noted between duanyu1842RC expression and histological markers of severity and overall survival data. CONCLUSIONS:The relevance of duanyu1842RC expression in pancreato-biliary carcinomas though may still be important for therapeutic decision making, it is not responsible for peritumoral stromal desmoplasia in these tumors and it does not have any significant prognostic implication.

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