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SP-A and TLR4 localization in lung tissue of SM-exposed patients.

Int. Immunopharmacol.2020 Mar;80:105936. Epub 2019 Nov 11
Sara Ghaffarpour 1 , Abbas Foroutan 2 , Nayere Askari 3 , Fatemeh Mashhadi Abbas 4 , Eisa Salehi 5 , Maryam Nikoonejad 1 , Mohammad Mehdi Naghizadeh 6 , Maryam Eskandarian 7 , Keivan Gohari Moghadam 8 , Hassan Mohammad Hosseini Akbari 9 , Mohammad Ebrahim Yarmohammadi 10 , Tooba Ghazanfari 11
Sara Ghaffarpour 1 , Abbas Foroutan 2 , Nayere Askari 3 , Fatemeh Mashhadi Abbas 4 , Eisa Salehi 5 , Maryam Nikoonejad 1 , Mohammad Mehdi Naghizadeh 6 , Maryam Eskandarian 7 , Keivan Gohari Moghadam 8 , Hassan Mohammad Hosseini Akbari 9 , Mohammad Ebrahim Yarmohammadi 10 , Tooba Ghazanfari 11
+ et al

[No authors listed]

Author information
  • 1 Immunoregulation Research Center, Shahed University, Tehran, Iran.
  • 2 Department of Physiology, Shaheed Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: sa.foroutan@sbu.ac.ir.
  • 3 Department of Biology, Faculty of Basic Sciences, Shahid Bahonar, University of Kerman, Kerman, Iran.
  • 4 Department of Oral & Maxillofacial Pathology, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • 5 Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • 6 Immunoregulation Research Center, Shahed University, Tehran, Iran; Non Communicable Diseases Research Center, Fasa University of Medical Science, Fasa, Iran.
  • 7 Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  • 8 Internal Medicine Department, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: kgohari@tums.ac.ir.
  • 9 Department of Pathology, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Traditional Medicine School, Tehran University of Medical Sciences, Tehran, Iran.
  • 10 Department of Otolaryngology, Shahed University, Tehran, Iran.
  • 11 Immunoregulation Research Center, Shahed University, Tehran, Iran; Department of Immunology, Shahed University, Tehran, Iran. Electronic address: ghazanfari@shahed.ac.ir.

摘要

INTRODUCTION:Long-term pulmonary complications are one of the major long-term consequences of sulfur mustard (SM) exposure. Toll-like receptor 4 (TLR4) involves in the pathogenesis of several pulmonary disorders. Surfactant protein-A (SP-A) regulates LPS-induced TLR4 localization and activation responses. However, the intensity and significance of TLR4 and SP-A expression by lung cells in SM-exposed patients is not clear. METHODS:The gene expression of TLR4 (through real-time PCR) and TLR4 and SP-A positive cells and alveolar type II cells, as SP-A producers, (using IHC) were assessed in formalin fixed paraffin embedded (FFPE) specimens from SM-exposed (n = 17), and non-SM exposed individuals (n = 12). RESULTS:TLR4 gene expression did not change between study groups. However, its cell surface presentation was significantly reduced in SM-exposed patients and particularly in which with constrictive bronchiolitis compared with the control group (P < 0.001 and P = 0.002, respectively). Frequency of alveolar type II cells was lower in the case group rather than the control group while the number of SP-A positive cells did not alter. CONCLUSIONS:These findings suggest that reduced TLR4 cell surface presentation may have anti-inflammatory function and SP-A may have a critical role in regulation of inflammatory responses in SM-exposed patients. Further investigation on other possible mechanisms involved in TLR4 internalization maybe help to illustrate the modulatory or inflammatory activity of TLR4 in these patients.

KEYWORDS: Alveolar type II cells, Constrictive bronchiolitis, Mustard gas, Surfactant protein-A (SP-A), Toll-like receptor 4 (TLR4)

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