例如:"lncRNA", "apoptosis", "WRKY"

Adamts18 deficiency in zebrafish embryo causes defective trunk angiogenesis and caudal vein plexus formation.

Biochem Biophys Res Commun. 2020 Jan 22;521(4):907-913. Epub 2019 Nov 09
Tiantian Lu 1 , Tianhao Zhang 1 , Caiyun Wang 1 , Ning Yang 1 , Yi-Hsuan Pan 1 , Suying Dang 2 , Wei Zhang 3
Tiantian Lu 1 , Tianhao Zhang 1 , Caiyun Wang 1 , Ning Yang 1 , Yi-Hsuan Pan 1 , Suying Dang 2 , Wei Zhang 3
+ et al

[No authors listed]

Author information
  • 1 Key Laboratory of Brain Functional Genomics (Ministry of Education and Shanghai), School of Life Sciences, East China Normal University, Shanghai, China.
  • 2 Department of Biochemistry and Molecular Cell Biology, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Research Center for Model Organisms, Shanghai, 201203, China. Electronic address: suyingdang@shsmu.edu.cn.
  • 3 Key Laboratory of Brain Functional Genomics (Ministry of Education and Shanghai), School of Life Sciences, East China Normal University, Shanghai, China. Electronic address: wzhang@sat.ecnu.edu.cn.

摘要


ADAMTS (A Disintegrin and Metalloproteinase with Thrombospondin type I motifs) enzymes play an important role in various morphogenesis processes. To determine the functions of Adamts18 in the early stages of organogenesis, we created Adamts18 deficient zebrafish using morpholino antisense oligonucleotides (MO) to generate exon 3 skipped adamts18 mRNA transcripts. Results showed that Adamts18 deficiency in zebrafish embryos caused developmental defects, including expanded brain ventricle and hindbrain edema, eye defects, and accumulation of blood in the caudal vein. Adamts18 deficiency also led to impaired trunk angiogenesis and formation of the caudal vein plexus (CVP). Consequently, Adamts18 deficient zebrafish embryos exhibited incomplete formation of intersegment vessels (ISVs), disruption of the honeycomb structure of CVP, and reduced CVP area and loop number. Furthermore, Adamts18 deficiency resulted in impaired blood circulation in major trunk, caudal vein (CV), and common cardinal vein (CCV). These aberrant vascular phenotypes in mutant zebrafish embryos were shown to be associated with a decreased expression of multiple angiogenesis-related signaling genes, including slit/robo, dll4/Notch, cox2, and fgfr. These findings indicate the critical role of Adamts18 in the early stages of vascular network development.

KEYWORDS: ADAMTS18, Angiogenesis, Blood circulation, Organogenesis, Zebrafish