Diagnostic Significance of miR-937 in Peripheral Blood Mononuclear Cells of Kawasaki Disease.

Beckground: The current study aims to investigate whether miR-937 can be used as a diagnostic biomarker in peripheral blood mononuclear cells (PBMCs) for children with Kawasaki disease (KD) with or without coronary artery dilation (CAD). METHODS:Gene chip technology was used to screen miRNAs differentially expressed between KD children and normal healthy children. Furthermore, real time PCR was carried out to validate the expression of miR-937 in 50 children with KD (25 cases with and 25 cases without CAD) and 25 healthy children. Meanwhile, target genes of miR-937 were analyzed using TargetScan and dual luciferase reporter assay. RESULTS:First, 20 miRs with significantly differentially expressed mononuclear cell (PBMCs) in peripheral blood between children with KD and normal healthy children were identified by gene chip technology. Real time PCR analysis validated that the expression of miR-937 decreased most significantly among all differentially expressed miRNAs. Secondly, miR-937 was down-regulated significantly before treatment with gamma globulin (IVIG), while its expression was significantly up-regulated after IVIG treatment. In addition, the expression of miR-937 in KD children with CAD was significantly lower than that of KD children without CAD. Person's correlation assay showed that miR-937 negatively correlated with CAD. Dual luciferase reporter assay indicated that IL-1β was a target gene of miR-937. CONCLUSIONS:In summary, miR-937 in PBMCs was involved in the occurrence and development of KD, which provides new ideas for the prevention and treatment of KD coronary artery dilation.

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