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Interleukin-27 is abrogated in gastric cancer, but highly expressed in other Helicobacter pylori-associated gastroduodenal diseases.

Helicobacter. 2020 Feb;25(1):e12667. doi:10.1111/hel.12667. Epub 2019 Nov 08
Gifone A Rocha 1 , Fabrício F de Melo 2 , Mônica M D A Cabral 3 , Breno B de Brito 2 , Filipe A F da Silva 2 , Dulciene M M Queiroz 1
Gifone A Rocha 1 , Fabrício F de Melo 2 , Mônica M D A Cabral 3 , Breno B de Brito 2 , Filipe A F da Silva 2 , Dulciene M M Queiroz 1
+ et al

[No authors listed]

Author information
  • 1 Laboratory of Research in Bacteriology, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • 2 Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Brazil.
  • 3 Department of Pathology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

摘要


BACKGROUND:IL-27 has dual roles in the immune response either stimulating Th1 or inhibiting Th17 cells. Because there is a particular link of IL-23/Th17 axis in the development of cancer and IL-27 has been considered a potential treatment for cancer, we evaluated the gastric and serum concentrations of IL-27 in two mutually exclusive Helicobacter pylori-associated diseases, gastric cancer (GC) and duodenal ulcer (DU). MATERIAL AND METHODS:We prospectively studied 110 H pylori-positive patients and 40 healthy blood donors. Serum and gastric concentrations of IL-27 and cytokines of the Th1/Th17 cells were assessed by ELISA. RESULTS:IL-27 was not detected in GC patients, but the cytokine concentration was very high in the patients with DU. IL-27 was also detected in the gastritis patients and in the H pylori-positive blood donors. IL27RA mRNA expression in peripheral blood mononuclear cells, evaluated by rt-PCR, was stimulated by H pylori strains. The cytokine concentration positively correlated with the Th1 and negatively with Th17 cell representative cytokine levels. Gastric IL-27 concentrations were positively correlated with increased degree of mononuclear and polymorphonuclear cells on the antral gastric mucosa of DU patients in consonance with the DU gastritis pattern. IL-12p70 and IFN-γ gastric concentrations were significantly higher in DU than in GC. Conversely, gastric concentrations of Th17 cell-associated cytokines (IL-1β, IL-6, IL-17A, IL-23, and TGF-β) were significantly higher in GC than in DU patients. CONCLUSION:Although H pylori infection is able to elicit IL-27 and IL-27Rα secretion, DU and GC have diametrically opposed cytokine patterns.

KEYWORDS: Helicobacter pylori , IL-27, Th1, Th17 cytokines, duodenal ulcer, gastric cancer