miR-98-5p promotes apoptosis and inhibits migration and cell growth in papillary thyroid carcinoma through Bax/Caspase-3 by HMGA2.

BACKGROUND:The aims of this study were to investigate the function and mechanism of miRNA-98-5p in papillary thyroid carcinoma. METHODS:Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the expression of miRNA-98-5p in papillary thyroid carcinoma. Western blotting and caspase-3/9 activity levels, flow cytometric analysis, cell migration assays, DAPI assay, cell proliferation assay, and LDH activity levels were used in this study. RESULTS:In patient with papillary thyroid carcinoma, miRNA-98-5p was reduced, and HMGA2 was increased. Downregulation of miRNA-98-5p promoted the cell growth, inhibited apoptosis, and induced HMGA2 protein expression in papillary thyroid carcinoma cell via activation of HMGA2. Overexpression of miRNA-98-5p inhibited the cell growth, induced apoptosis, and suppressed HMGA2 protein expression in papillary thyroid carcinoma cell through the suppression of HMGA2. Si-HMGA2 inhibited the effects of anti-miRNA-98-5p on cell growth of papillary thyroid carcinoma. CONCLUSION:Therefore, these results suggested the regulation of HMGA2 suppresses proliferation of papillary thyroid carcinoma through miRNA-98-5p.

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