The circular RNA ZNF292 alleviates OGD-induced injury in H9c2 cells via targeting BNIP3.

The research aims to explore the roles and regulatory mechanisms of the circular RNA (circRNA) ZNF292 (circZNF292) in OGD-induced damage in H9c2 cells. The H9c2 cells were treated by OGD and/or transfected with circZNF292, si-circZNF292, pc-Bcl-2/adenovirus E1B-19 kDa-interacting protein 3 (BNIP3) or corresponding controls. Cell viability was detected with the CCK-8. The protein expression levels of the Bax, caspase-3, Beclin-1, p62, LC3, BNIP3, Wnt3a, β-catenin and mammalian target of rapamycin (mTOR) were individually determined via western blot. qRT-PCR was used to examine the circZNF292 expression level. The apoptotic rate was determined by the Annexin V-FITC/PI with flow cytometer. The production of the circZNF292 was promoted by OGD. Abundant circZNF292 released OGD-induced damage by up-regulating cell viability and Wnt3a/β-catenin or mTOR proteins, but down-regulating apoptosis and autophagy. circZNF292 had an opposite effect on these elements mentioned above. Besides, BNIP3 was negatively adjusted by the circZNF292. The BNIP3 overproduction destroyed the protective effect of circZNF292 on H9c2. circZNF292 released OGD-induced damage in the H9c2 cells by targeting the BNIP3 through Wnt/β-catenin and mTOR activation.

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