[No authors listed]
The present study was aimed to observe the protective effect of rapamycin on cognitive dysfunction induced by sevoflurane in aged rats and its effect on autophagyârelated proteins, and to investigate the regulatory mechanism of the Tollâlike receptor 4/myeloid differentiation primary response 88/nuclear factorâκB (TLR4/MyD88/NFâκB) signaling pathway. Fifty SpragueâDawley rats were randomly assigned to a control group, a sevoflurane group, a rapamycin pretreatment group, a TLR4 inhibitor group and a 3MA autophagy inhibitor group. A water maze test was used to evaluate the cognition and memory of rats. Hematoxylin and eosin (H&E) staining was performed to observe pathological changes of brain tissue. A TUNEL assay was used to detect the apoptosis of brain tissue. ELISA was used to assess changes in brain injury markers and inflammatory factors. A western blot assay or quantitative reverse transcription PCR (RTâqPCR) were performed to determine the expression of autophagyârelated proteins and the TLR4/MyD88/NFâκB signaling pathway in brain tissue. The results revealed that rapamycin could improve cognitive dysfunction of aged rats induced by sevoflurane. Rapamycin was identified to play a therapeutic role, including mitigating brain tissue damage, inhibiting apoptosis, and activating autophagy in a sevofluraneâtreated aged rat model. This function of rapamycin was demonstrated to depend on the TLR4/MyD88/NFâκB signaling pathway.
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