Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling.
[No authors listed]
摘要
Extracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and size of EVs as well as decreased the abundance of EVs. In contrast, strains with deletions in cell wall biosynthesis genes, produced more EVs than wildtype. Proteomic analysis highlighted the depletion of ESCRT components and enrichment of cell wall remodelling enzymes, glucan synthase subunit Fks1 and chitin synthase Chs3, in yeast EVs. Interestingly, EVs containing Fks1 and Chs3 rescued the yeast cells from antifungal molecules. However, EVs from fks1â or chs3â or the vps23âchs3â double knockout strain were unable to rescue the yeast cells as compared to vps23â EVs. Overall, we have identified a potential role for yeast EVs in cell wall remodelling.
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基因功能
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原始数据
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文献解读
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