例如:"lncRNA", "apoptosis", "WRKY"

Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration.

Brain Behav. 2019 Sep;9(9):e01378. doi:10.1002/brb3.1378. Epub 2019 Jul 31
Xuan Li 1 , Ian R Davis 1 , Olivia M Lofaro 2 , Jianjun Zhang 3 , Raffaello Cimbro 4 , F Javier Rubio 2
Xuan Li 1 , Ian R Davis 1 , Olivia M Lofaro 2 , Jianjun Zhang 3 , Raffaello Cimbro 4 , F Javier Rubio 2
+ et al

[No authors listed]

Author information
  • 1 Department of Psychology, University of Maryland College Park, College Park, Maryland.
  • 2 Intramural Research Program, NIDA, NIH, DHHS, Baltimore, Maryland.
  • 3 CAS Key Laboratory of Mental Health, Institute of Psychology, Beijing, China.
  • 4 Division of Rheumatology, School of Medicine, Johns Hopkins University, Baltimore, Maryland.

摘要


BACKGROUND:Methamphetamine (Meth) seeking progressively increases after withdrawal (incubation of Meth craving). We previously demonstrated a role of anterior intralaminar nucleus of thalamus (AIT) to dorsomedial striatum (DMS) projections in this incubation. Here, we examined molecular alterations in DMS and AIT neurons activated (identified by neuronal activity marker Fos) during "incubated" Meth-seeking relapse test after prolonged withdrawal. METHODS:We trained male rats to self-administer Meth or saline (control condition) for 10 days (6 hr/day). Using fluorescence-activated cell sorting, we examined gene expression in Fos-positive (activated during a 2-hr relapse test) and Fos-negative (nonactivated) DMS and AIT neurons. RESULTS:In DMS, we found increased mRNA expressions of immediate early genes (IEGs) (Arc, Egr1, Npas4, Fosb), Trkb, glutamate receptors subunits (Gria3, Grin1, Grin2b, Grm1), and epigenetic enzymes (Hdac3, Hdac5, Crebbp) in Fos-positive neurons, compared with Fos-negative neurons. In AIT, we found that fewer genes (Egr1, Fosb, TrkB, Grin1, and Hdac5) exhibited increased mRNA expression in Fos-positive neurons. Unexpectedly, in both brain regions, gene alterations described above also occurred in drug-naïve saline self-administration control rats. CONCLUSIONS:These results demonstrated that transcriptional regulations in Fos-positive neurons activated during the relapse tests are brain region-specific but are not uniquely associated with drug exposure during the self-administration training.

KEYWORDS: Fos, anterior intralaminar nucleus of thalamus, dorsal striatum, fluorescence-activated cell sorting, incubation of drug craving, methamphetamine relapse