Long non-coding RNA PTTG3P functions as an oncogene by sponging miR-383 and up-regulating CCND1 and PARP2 in hepatocellular carcinoma.

BACKGROUND:Emerging evidence indicates that Long non-coding RNAs (LncRNAs) and microRNAs (miRNAs) play crucial roles in tumor progression, including hepatocellular carcinoma (HCC). However, whether there is a crosstalk between LncRNA pituitary tumor-transforming 3 and miR-383 in HCC remains unknown. This study is designed to explore the underlying mechanism by which LncRNA sponges miR-383 during HCC progression. METHODS:qPCR and Western blot were used to analyze LncRNA miR-383 and other target genes' expression. CCK-8 assay was performed to examine cell proliferation. Annexin V-PE/PI and PI staining were used to analyze cell apoptosis and cell cycle distribution by flow cytometry, respectively. Transwell migration and invasion assays were used to examine cell migration and invasion abilities. An in vivo xenograft study was performed to detect tumor growth. Luciferase reporter assay and RNA pull-down assay were carried out to detect the interaction between miR-383 and LncRNA RIP was carried out to detect whether duanyu1547G3P and miR-383 were enriched in Ago2-immunoprecipitated complex. RESULTS:In this study, we found that duanyu1547G3P was up-regulated in HCC tissues and cells. Functional experiments demonstrated that knockdown of duanyu1547G3P inhibited cell proliferation, migration and invasion, and promoted cell apoptosis, acting as an oncogene. Mechanistically, duanyu1547G3P upregulated the expression of miR-383 targets Cyclin D1 (CCND1) and poly ADP-ribose polymerase 2 by sponging miR-383, acting as a competing endogenous RNA (ceRNA). The axis modulated HCC phenotypes. Moreover, duanyu1547G3P also affected the PI3K/Akt signaling pathway. CONCLUSION:The data indicate a novel duanyu1547G3P-miR-383-CCND1/Pduanyu372 axis in HCC tumorigenesis, suggesting that duanyu1547G3P may be used as a potential therapeutic target in HCC.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}