Exposure to cadmium causes inhibition of otolith development and behavioral impairment in zebrafish larvae.

Otolith consisting largely of calcium carbonate, fibrous and proteins, is vital for maintaining body balance and/or hearing of fish. The formation of otolith involves Ca²⁺ transport and deposition. In the present study, we investigated the effects of Cd²⁺ on otoliths development by using zebrafish embryos as model. The results showed that exposure to Cd²⁺ inhibited the utricular and saccular otoliths growth, indicated by reduced lateral areas. Swimming speeds were reduced and a losing balance control was observed in Cd²⁺ exposed larvae. The genes related to Ca²⁺ transport (e.g. plasma membrane Ca²⁺-ATPase isoform 2, pmca2; Ca²⁺-ATPase isoform 2, atp2b1a) and regulation (e.g. parathyroid hormone ligand type-1, pth1; stanniocalcin isoform 1, stc1) were significantly downregulated. However, the adverse effects of Cd²⁺ on otoliths growth and swimming activity can be protected by supplementation of Ca²⁺ in exposure medium. Body burden of Cd²⁺ in larvae was reduced upon the supplement with Ca²⁺. The overall results suggest that exposure to Cd²⁺ can inhibit influx of Ca²⁺, leading to less deposition of CaCO₃ for otolith growth, and finally result in impaired balance control and swimming activity in zebrafish larvae.

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