[No authors listed]
Dysregulation of microRNAâ3613â3p (miRâ3613â3p) was previously reported in endothelial cells (ECs) during heat stress. The aim of the present study was to investigate the precise role of miRâ3613â3p in heat stress. In the present study, potential gene targets of miRâ3613â3p in heatâtreated ECs were assessed, and the potential effects of miRâ3613â3p were determined using Gene Ontology enrichment analysis. Kyoto Encyclopedia of Genes and Genomes pathway analysis was used to identify signaling pathways that may be affected by miRâ3613â3p in heatâtreated cells. Reverse transcriptionâquantitative PCR, western blotting and annexin VâFITC/propidium iodide staining were performed to detect miRNA expression, protein expression and apoptosis, respectively. Luciferase gene reporter assay was performed to evaluate the association between miRâ3613â3p and mitogenâactivated protein kinase kinase kinase 2 (MAP3K2). Bioinformatics analysis revealed 865 potential gene targets for miRâ3613â3p and a series of functions and pathways in heatâtreated ECs. 'Negative regulation of apoptotic process' was identified as a potential function of miRâ3613â3p. In addition, functional analysis confirmed the downregulated expression levels of miRâ3613â3p in ECs during heat stress, which was accompanied by an increase in apoptosis; restoration of miRâ3613â3p expression inhibited apoptosis. MAP3K2 protein was demonstrated to be upregulated in heatâtreated ECs, and overexpression of miRâ3613â3p reduced MAP3K2 expression levels. Additionally, MAP3K2 was targeted by miRâ3613â3p. These results indicated that miRâ3613â3p may have complicated roles in ECs under heat stress. miRâ3613â3p may serve an important role in the apoptosis of heatâtreated ECs, and this effect may be partly achieved by targeting MAP3K2.
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