[No authors listed]
MicroRNAs (miRNAs) have been found to play important regulatory roles in certain neurodegenerative diseases. The aim of the present study was to investigate the effect of miRNAâ153 (miRâ153) on the neural differentiation of HTâ22 cells. Overexpression of miRâ153 induced the differentiation of HTâ22 cells, increasing the number of protrusions and branches, reducing the S phase distribution of the cell cycle, and attenuating the cell proliferation rate as determined using the Cell Counting Kitâ8 assay. Furthermore, miRâ153 increased the expression of neuronâspecific γâenolase (NSE), neuronal nuclei (NeuN), and Nâethylmaleimideâsensitive fusion attachment protein 23 (SNAP23) and SNAP25 at the transcriptional and protein level by PCR and western blot analysis. Moreover, miRâ153 caused obvious upregulation of peroxiredoxin 5 (PRX5), which has been found to protect neural cells from death and apoptosis. miRâ153 promoted neural differentiation and protected neural cells by upregulating the neuron markers γâenolase, neuronal nuclei, and the functional proteins SNAP23, SNAP25 and PRX5. Therefore, miRâ153 may be a potential target for the treatment of certain neurodegenerative diseases.
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