TSPAN9 and EMILIN1 synergistically inhibit the migration and invasion of gastric cancer cells by increasing TSPAN9 expression.

BACKGROUND:Globally, the incidence and mortality rates of gastric cancer are high, and its poor prognosis is closely related to tumor recurrence and metastasis. Therefore, the molecular mechanisms associated with the migration and invasion of gastric cancer cells are important for gastric cancer treatment. Previously, has been reported to inhibit gastric cancer cell migration; however, the underlying molecular mechanism remains unclear. METHODS:Human gastric adenocarcinoma cell lines, SGC7901 and AGS, were cultured in vitro. Tduanyu1842N9 expression was determined by RT-PCR, western blot analysis, and immunohistochemistry in gastric cancer and tumor-adjacent tissues. Following the over-expression and knockdown of wound healing and cell invasion assays were performed and EMT-related protein expression was evaluated to analyze the invasion and migration of gastric cancer cells. Tduanyu1842N9 expression and the invasion and metastasis of gastric cancer cells were observed by the functional assays following EMILIN1 over-expression. RESULTS:Inhibiting Tduanyu1842N9 expression significantly promoted the migration and invasion of gastric cancer cells. In addition, immunofluorescence co-localization and co-immunoprecipitation analysis revealed closely related expression of EMILIN1 and Moreover, EMILIN1 can synergistically boost the tumor suppressive effect of Tduanyu1842N9, which may be produced by promoting Tduanyu1842N9 expression. CONCLUSIONS:We have demonstrated that EMILIN1 induces anti-tumor effects by up-regulating Tduanyu1842N9 expression in gastric cancer. Hence, membrane proteins Tduanyu1842N9 and EMILIN1 may represent novel therapeutic targets for the treatment of gastric cancer.

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