例如:"lncRNA", "apoptosis", "WRKY"

Ribonuclease 7 Shields the Kidney and Bladder from Invasive Uropathogenic Escherichia coli Infection.

J Am Soc Nephrol. 2019 Aug;30(8):1385-1397. Epub 2019 Jun 25
Tad Eichler 1 , Kristin Bender 1 , Matthew J Murtha 2 , Laura Schwartz 1 , Jackie Metheny 3 , Lindsey Solden 4 , Robert M Jaggers 4 , Michael T Bailey 4 , Sudipti Gupta 1 , Claudia Mosquera 3 , Christina Ching 5 , Krista La Perle 6 , Birong Li 3 , Brian Becknell 7 , John David Spencer 7
Tad Eichler 1 , Kristin Bender 1 , Matthew J Murtha 2 , Laura Schwartz 1 , Jackie Metheny 3 , Lindsey Solden 4 , Robert M Jaggers 4 , Michael T Bailey 4 , Sudipti Gupta 1 , Claudia Mosquera 3 , Christina Ching 5 , Krista La Perle 6 , Birong Li 3 , Brian Becknell 7 , John David Spencer 7
+ et al

[No authors listed]

Author information
  • 1 Nephrology and Urology Research Affinity Group.
  • 2 Department of Pediatrics, The Ohio State University College of Medicine, Columbus, Ohio; and.
  • 3 Centers for Clinical and Translational Research and.
  • 4 Microbial Pathogenesis, The Research Institute at Nationwide Children's, Columbus, Ohio.
  • 5 Departments of Pediatric Surgery and.
  • 6 Comparative Pathology and Mouse Phenotyping Shared Resource, The Ohio State University College of Veterinary Medicine, Columbus, Ohio.
  • 7 Pediatrics, Nationwide Children's, Columbus, Ohio.

摘要


BACKGROUND:Evidence suggests that antimicrobial peptides, components of the innate immune response, protect the kidneys and bladder from bacterial challenge. We previously identified ribonuclease 7 (RNase 7) as a human antimicrobial peptide that has bactericidal activity against uropathogenic Escherichia coli (UPEC). Functional studies assessing RNase 7's contributions to urinary tract defense are limited. METHODS:To investigate RNase 7's role in preventing urinary tract infection (UTI), we quantified urinary RNase 7 concentrations in 29 girls and adolescents with a UTI history and 29 healthy female human controls. To assess RNase 7's antimicrobial activity in vitro in human urothelial cells, we used siRNA to silence urothelial RNase 7 production and retroviral constructs to stably overexpress RNase 7; we then evaluated UPEC's ability to bind and invade these cells. For RNase 7 in vivo studies, we developed humanized RNase 7 transgenic mice, subjected them to experimental UTI, and enumerated UPEC burden in the urine, bladder, and kidneys. RESULTS:Compared with controls, study participants with a UTI history had 1.5-fold lower urinary RNase 7 concentrations. When RNase 7 was silenced in vitro, the percentage of UPEC binding or invading human urothelial cells increased; when cells overexpressed RNase 7, UPEC attachment and invasion decreased. In the transgenic mice, we detected RNase 7 expression in the kidney's intercalated cells and bladder urothelium. RNase 7 humanized mice exhibited marked protection from UPEC. CONCLUSIONS:These findings provide evidence that RNase 7 has a role in kidney and bladder host defense against UPEC and establish a foundation for investigating RNase 7 as a UTI prognostic marker or nonantibiotic-based therapy. Copyright © 2019 by the American Society of Nephrology.

KEYWORDS: antimicrobial peptides, intercalated cells, ribonuclease 7, urinary tract infection, urothelium