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Co-expression of carcinoembryonic antigen-related cell adhesion molecule 6 and 8 inhibits proliferation and invasiveness of breast carcinoma cells.

Clin. Exp. Metastasis. 2019 Oct;36(5):423-432. Epub 2019 Jun 20
Erina Iwabuchi 1 , Yasuhiro Miki 2 , Yoshiaki Onodera 3 , Yukiko Shibahara 4 , Kiyoshi Takagi 5 , Takashi Suzuki 5 , Takanori Ishida 6 , Hironobu Sasano 7
Erina Iwabuchi 1 , Yasuhiro Miki 2 , Yoshiaki Onodera 3 , Yukiko Shibahara 4 , Kiyoshi Takagi 5 , Takashi Suzuki 5 , Takanori Ishida 6 , Hironobu Sasano 7
+ et al

[No authors listed]

Author information
  • 1 Japan Society for the Promotion of Science, Tokyo, Japan.
  • 2 Department of Disaster Obstetrics and Gynecology, International Research Institute of Disaster Science (IRIDes), Tohoku University, Sendai, Japan.
  • 3 Department of Pathology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.
  • 4 Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, Canada.
  • 5 Department of Pathology and Histotechnology, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • 6 Department of Breast and Endocrine Surgical Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • 7 Department of Pathology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan. hsasano@patholo2.med.tohoku.ac.jp.

摘要

The carcinoembryonic antigen-related cell adhesion molecule (CEACAM) 6 and CEACAM8 form heterodimers and exert their effects. Therefore, we examined the effects of CEACAM6 and CEACAM8 co-expression in breast cancer. We first studied CEACAM6/8 expression using immunohistochemistry in 109 patients with breast cancer. We then established MCF-7 cells that were stably transfected with CEACAM8 and studied cell proliferation, invasion and adhesion. The number of CEACAM6 and CEACAM8 double-positive breast carcinoma cells significantly increased in patients with low histopathological grade and stage. Proximity ligation assay (PLA) confirmed high CEACAM6/8 expression in MCF-7 cells. CEACAM6/8 expression promoted the adhesion of MCF-7 cells to endothelial cell monolayers but inhibited their invasion and proliferation. Furthermore, CEACAM6 status in carcinoma cells was significantly higher in bone than in lung metastases. CEACAM6/8 expression is associated with the inhibition of vascular invasion and cell proliferation. CEACAM6 expression was also considered to be involved in bone metastases of breast cancer. This is the first study to demonstrate the possible role of CEACAM6/8 heterodimer and CEACAM6 expression in breast cancer patients.

KEYWORDS: Breast cancer, CEACAM6, CEACAM8, Cancer metastasis, Protein–protein interaction

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