[No authors listed]
We examined interactions between the 83 kDa heat-shock protein (Hsp83) and hsrÏ long noncoding RNAs (lncRNAs) in hsrÏ66 Hsp90GFP homozygotes, which almost completely lack hsrÏ lncRNAs but over-express Hsp83. All +/+; hsrÏ66 Hsp90GFP progeny died before the third instar. Rare Sp/CyO; hsrÏ66 Hsp90GFP reached the third instar stage but phenocopied l(2)gl mutants, becoming progressively bulbous and transparent with enlarged brain and died after prolonged larval life. Additionally, ventral ganglia too were elongated. However, hsrÏ66 Hsp90GFP/TM6B heterozygotes, carrying +/+ or Sp/CyO second chromosomes, developed normally. Total RNA sequencing (+/+, +/+; hsrÏ66/hsrÏ66, Sp/CyO; hsrÏ66/ hsrÏ66, +/+; Hsp90GFP/Hsp90GFP and Sp/CyO; hsrÏ66 Hsp90GFP/hsrÏ66 Hsp90GFP late third instar larvae) revealed similar effects on many genes in hsrÏ66 and Hsp90GFP homozygotes. Besides additive effect on many of them, numerous additional genes were affected in Sp/CyO; hsrÏ66 Hsp90GFP larvae, with l(2)gl and several genes regulating the central nervous system being highly down-regulated in surviving Sp/CyO; hsrÏ66 Hsp90GFP larvae, but not in hsrÏ66 or Hsp90GFP single mutants. Hsp83 and several omega speckle-associated hnRNPs were bioinformatically found to potentially bind with these gene promoters and transcripts. Since Hsp83 and hnRNPs are also known to interact, elevated Hsp83 in an altered background of hnRNP distribution and dynamics, due to near absence of hsrÏ lncRNAs and omega speckles, can severely perturb regulatory circuits with unexpected consequences, including down-regulation of tumoursuppressor genes such as l(2)gl.
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