[No authors listed]
Stanniocalcin 2 (STC2), a secretory glycoprotein hormone, regulates many biological processes including cell proliferation, apoptosis, tumorigenesis and atherosclerosis. However, the effect of STC2 on proliferation, migration and epithelialâmesenchymal transition (EMT) progression in human colorectal cancer (CRC) cells remains poorly understood. The expression level of STC2 was determined by quantitative realâtime polymerase chain reaction (qPCR) and western blot analysis. Cell Counting Kitâ8 (CCKâ8) was used to detect the viability of SW480 cells. The invasion and migration of cells were identified by wound healing and Transwell assays. The mRNA and protein expression levels of βâcatenin, matrix metalloproteinase (MMP)â2, MMPâ9, Eâcadherin and vimentin were assessed by qPCR and western blot analysis. In the present study, it was demonstrated that STC2 was highly expressed in the CRC cell lines. After silencing of STC2, the cell viability, migration and invasion were significantly reduced. Silencing of STC2 in the CRC Sw480 cells increased the expression of Eâcadherin and decreased the expression of vimentin, MMPâ2 and MMPâ9, compared to those in the normal and empty vector group. Furthermore, the expression of βâcatenin in the STC2 gene silenced group was suppressed, and the expression of βâcatenin was reversed by Wnt activator, SB216763. These results demonstrated that STC2 participates in the development and progression of CRC by promoting CRC cell proliferation, survival and migration and activating the Wnt/βâcatenin signaling pathway.
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