[No authors listed]
An increasing body of evidence has demonstrated that microRNA (miR) deregulation serves pivotal roles in tumor progression and metastasis. However, the function of miRâ379 in lung cancer remains understudied, particularly in nonâsmall cell lung cancer (NSCLC). Bioinformatics and luciferase reporter analyses confirmed that conserved helixâloopâhelix ubiquitous kinase (CHUK) is a target of miRâ379, which may directly bind to the 3'âuntranslated region of CHUK and significantly downregulate its expression in NSCLC cells. Transwell assays were used to evaluate the role of miRâ379 in cell migration and invasion, and western blotting was used to address the association between miRâ379 and epithelialâmesenchymal markers, including Eâcadherin, cytokeratin and Vimentin. In the present study, miRâ379 expression in NSCLC tissues and cell lines was downregulated, which may be associated with the poor survival of patients with NSCLC. miRâ379 may act as a tumor suppressor in NSCLC, potentially by suppressing cell growth and proliferation, delaying G1âS transition, enhancing cell apoptosis and suppressing NSCLC cell migration and invasion. Furthermore, it was also observed that CHUK may function as an oncogene, and downregulation of CHUK induced by miRâ379 may partially rescue the malignant characteristics of tumors, indicating that miRâ379 may be suppressed in tumorigenesis. The overexpression of miRâ379 may prevent the growth of NSCLC tumors via CHUK suppression and the downstream nuclear factorâκB pathway. The results of the present study demonstrated that miRâ379 may act as a tumor suppressor, and may constitute a potential biomarker and a promising therapeutic agent for the treatment for NSCLC.
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