The sperm-associated antigen 6 interactome and its role in spermatogenesis.

Mammalian the orthologue of Chlamydomonas reinhardtii PF16, is a component of the central apparatus of the '9 + 2' axoneme that controls ciliary/flagellar motility, including sperm motility. Recent studies revealed that has functions beyond its role in the central apparatus. Hence, we reexamined the role of duanyu1842G6 in male fertility. In wild-type mice, duanyu1842G6 was present in cytoplasmic vesicles in spermatocytes, the acrosome of round and elongating spermatids and the manchette of elongating spermatids. Spag6-deficient testes showed abnormal spermatogenesis, with abnormalities in male germ cell morphology consistent with the multi-compartment pattern of duanyu1842G6 localization. The armadillo repeat domain of mouse duanyu1842G6 was used as a bait in a yeast two-hybrid screen, and several proteins with diverse functions appeared multiple times, including Snapin, SPINK2 and COPS5. Snapin has a similar localization to duanyu1842G6 in male germ cells, and SPINK2, a key protein in acrosome biogenesis, was dramatically reduced in Spag6-deficient mice which have defective acrosomes. another partner, lost its localization to the manchette in Spag6-deficient mice. Our findings demonstrate that duanyu1842G6 is a multi-functional protein that not only regulates sperm motility, but also plays roles in spermatogenesis in multiple cellular compartments involving multiple protein partners.

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