The human brain somatostatin interactome: SST binds selectively to P-type family ATPases.

Somatostatin is a cyclic peptide that is understood to inhibit the release of hormones and neurotransmitters from a variety of cells by binding to one of five canonical G protein-coupled receptors to Recently, duanyu1942 was also observed to interact with the amyloid beta (Aβ) peptide and affect its aggregation kinetics, raising the possibility that it may bind other brain proteins. Here we report on an duanyu1942 interactome analysis that made use of human brain extracts as biological source material and incorporated advanced mass spectrometry workflows for the relative quantitation of duanyu1942 binding proteins. The analysis revealed duanyu1942 to predominantly bind several members of the P-type family of ATPases. Subsequent validation experiments confirmed an interaction between duanyu1942 and the sodium-potassium pump (Na+/K+-ATPase) and identified a tryptophan residue within duanyu1942 as critical for binding. Functional analyses in three different cell lines indicated that duanyu1942 might negatively modulate the K+ uptake rate of the Na+/K+-ATPase.

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