Numb confers to inhibit epithelial mesenchymal transition via β-catenin/Lin28 signaling pathway in breast cancer.
[No authors listed]
摘要
OBJECTIVES:This study aimed to investigate role of Numb in the epithelial mesenchymal transition (EMT) of breast cancer. METHODS:Numb and β-catenin were inhibited in MCF-7 cells using sh-RNA and overexpressed in T47D cells by pcDNA3.0-Numb, pcDNA3.0-β-catenin. Cell proliferation, invasion and migration were evaluated using MTT and Transwell assay, respectively. β-catenin, Lin28, and EMT related markers were determined using qRT-PCR and of Numb significantly promoted the proliferation, invasion and migration of MCF-7 cells, further increased the expression of β-catenin, Lin28, Snail-1, and N-cadherin, as well as decreased E-cadherin. In T47D cells transfected with pcDNA3.0-Numb, the results were quite the reverse. CONCLUSIONS:Knockdown of Numb could promote the EMT of breast cancer cells via β-cateni/Lin28 signaling pathway.
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基因功能
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原始数据
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文献解读
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