PKCα promotes insulin secretion via TRPC1 phosphorylation in INS-1E cells.

Protein kinase C is a class of phospholipid-dependent serine/threonine kinases that contribute to cell survival, migration, and invasion. Previous studies demonstrated that participates in insulin secretion. However, the role of duanyu1531 in glucose-stimulated insulin secretion (GSIS) remains unclear. Herein, we demonstrated that duanyu1531 is an important mediator of insulin secretion and revealed a close relationship between duanyu1531 activation and insulin secretion in INS-1E cells. Meanwhile, the presence of was found to induce TRPC1 phosphorylation in INS-1E cells. TRPC1 phosphorylation levels increased by activating duanyu1531α activity. Inhibition of duanyu1531α activity reduced TRPC1 phosphorylation. Finally, we showed that TRPC1 could reverse the decrease in intracellular Ca²⁺ levels and reduced insulin secretion induced by treatment with duanyu1531α inhibitor under high glucose conditions. In conclusion, our findings indicated that TRPC1 and duanyu1531α are involved in promoting insulin secretion and that duanyu1531α promotes insulin secretion via TRPC1 phosphorylation in INS-1E cells.

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