microRNA-370 Promotes Cell Growth by Targeting WNK2 in Breast Cancer.

Aberrant expression of microRNA-370 (miR-370) has been found in many types of human cancers. In non-small cell lung cancer, miR-370 is a tumor suppressor with decreased expression. In prostate cancer, it is an oncogene with overexpression. Although upregulation of miR-370 was correlated with poor prognosis of breast cancer, the exact function of miR-370 is largely unclear. The roles of miR-370 in breast cancer are investigated, and its downstream targets are identified. miR-370 expression was detected by reverse transcription polymerase chain reaction in breast cancer cells. Cell growth curve and colony formation were monitored on overexpressing or knocking down miR-370. We further identified the target of miR-370 and the role of the target in tumorigenesis by using western blot, luciferase assay, and the xenograft mouse model. miR-370 is increased in breast cancer cells. Its overexpression promotes cell growth, whereas the knockdown of miR-370 suppresses cell growth. WNK2 is a downstream target of miR-370. Manipulation of WNK2 expression affects miR-370-mediated cell proliferation and tumor growth. Our data suggest that miR-370 acts as an oncogene by downregulating WNK2 in breast cancer, providing a molecular basis for clinical application of miR-370 as a potential biomarker.

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