High intensity interval training decreases the expressions of KIF5B and Dynein in Hippocampus of Wistar male rats.

Although exercise training (ET) with low to moderate intensity improves several physiological aspects of brain, the effects of high intensity interval training (HIIT) are less clear on brain plasticity and cytoplasmic transport. The present study examined the effects of HIIT on the gene and protein expressions of kinesin family member 5B (KIF5B) and Dynein in the Wistar male rat hippocampal tissue. Fourteen male Wistar rats were separated into 2 groups: (1) the training group (TG: n = 7) and (2) the control group (CG: n = 7). The exercise protocol was carried out on a rodent treadmill (5 days a week for 6 weeks). The protein contents of KIF5B and Dynein were determined by the immunohistochemical analysis. Moreover, the Real-Time polymerase chain reaction (Real-Time PCR) procedure was done to measure the KIF5B mRNA and Dynein mRNA expressions. It was observed that HIIT resulted in a significant decrease in the gene expressions of KIF5B and Dynein (P = 0.001), and also the results showed that HIIT leads to a significant decrease in KIF5B (P = 0.001) and Dynein (P = 0.02) protein content of the hippocampal tissue in comparison with sedentary rats. Our findings demonstrated that HIIT is associated with the down-regulation of gene and protein levels of KIF5B and Dynein in the rat hippocampal tissue, although the underlying mechanisms have remained unknown. These changes suggest that HIIT may have negative effects on both the anterograde and retrograde cytoplasmic transports because the cytoplasmic transport is mediated by KIF5B and Dynein.

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