MiR-539-3p promotes the progression of epithelial ovarian cancer by targeting SPARCL1.

OBJECTIVE:The aim of this study was to investigate the effect of microRNA-539-3p (miR-539-3p) on the development of epithelial ovarian cancer (EOC), and to explore the possible underlying mechanism. PATIENTS AND METHODS:A total of 40 paired EOC tissues and adjacent normal ovarian tissues were surgically resected in Hanchuan People's Hospital. Quantitative (qRT-PCR) was used to detect the expression of miR-539-3p in EOC tissues and cell lines. Targeted regulatory mechanism of miR-539-3p on protein 1 was identified by luciferase reporter and Western blot assays. Furthermore, the effects of axis on the malignant behaviors of EOC cells, including proliferation, invasion and migration abilities, were confirmed by cell counting kit-8 (CCK-8), transwell and scratch wound assays. RESULTS:QRT-PCR showed that the expression of miR-539-3p was significantly up-regulated in EOC tissues and cell lines. was a direct target of miR-539-3p in EOC cells. Overexpression of miR-539-3p significantly promoted the proliferation, migration and invasion of SKOV3 cells. Furthermore, co-transfection of miR-539-3p inhibitor and could remarkably restore the migration and invasion abilities of SKOV3 cells. CONCLUSIONS:MiR-539-3p acted as an oncogene in EOC by targeting axis, as a target for the treatment of EOC, might become a feasible and new method of tumor treatment.

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